Molecular survey of Canine Microfilariae Species in East-Azerbaijan province of Iran

نویسندگان

  • H. Babaei
  • H. Paykari
  • K. Esmaeilnia
  • L. Froghy
  • S. Sadegh-Eteghad
چکیده مقاله:

Filariasis in dogs is caused by several species of filariids. Because of importance of this infection in veterinary medicine and public health, it is necessary to carry out an epidemiological and cross sectional studies in various geographical areas and use of well-adapted diagnosis methods. In this study 205 capillary and whole blood samples were collected from doges in various counties of East-Azerbaijan province. Samples after preparation were examined by Knott`s test and light microscope for presence of microfiler. In molecular identification, Pan-filarial and species-specific PCR perimers was used to differentiate among Dirofilaria immitis, Dirofilaria repens, Acanthocheilonema reconditum, Acanthocheilonema dracunculoides, Brugia malayi and Brugia pahangi. Descriptive statistics were used for data analysis. Total infection prevalence with the microscopic evaluation was 77 (37.5 %) and in PCR test was 94 (45.8 %). The most common species of canine filarial parasite identified in this study was D. immitis 54 (57.4 %) followed by Acanthocheilonema species 40 (42.6 %). The molecular evidence on the sequence of the ITS-2 region provided strong evidence that the canine microfilariae discovered in this study belong to a novel species of Acanthocheilonema. Information about infection prevalence helps us to improve disease management practices in the studied area, apply new hygiene policy and reduce extra costs of therapeutic agents and PCR is a quick and accurate molecular genetics method for detection of filarial species.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

molecular survey of canine microfilariae species in east-azerbaijan province of iran

filariasis in dogs is caused by several species of filariids. because of importance of this infection in veterinary medicine and public health, it is necessary to carry out an epidemiological and cross sectional studies in various geographical areas and use of well-adapted diagnosis methods. in this study 205 capillary and whole blood samples were collected from doges in various counties of eas...

متن کامل

Molecular Identification of Nosema species in East Azerbaijan province, Iran

Nosema is a genus of microsporidia, which have significant negative impacts on honeybees. The aim of this study is the epidemiological evaluation and molecular characterization of Nosema spices in various counties of East-Azerbaijan province (Northwest of Iran). 387 samples were collected from colonies maintained in various counties of East-Azerbaijan province. Samples after preparation were ex...

متن کامل

molecular identification of nosema species in east azerbaijan province, iran

nosema is a genus of microsporidia, which have significant negative impacts on honeybees. the aim of this study is the epidemiological evaluation and molecular characterization of nosema spices in various counties of east-azerbaijan province (northwest of iran). 387 samples were collected from colonies maintained in various counties of east-azerbaijan province. samples after preparation were...

متن کامل

Prevalence of Oral Human Papilloma Virus in Healthy Individuals in East Azerbaijan Province of Iran

BACKGROUND Human papilloma virus causes benign and malignant abnormalities in different part of the body. The link between high risk types of HPV and some anogenital and aerodigestive tract cancer is well established. Oral HPV infection plays a role in developing oropharyngeal squamous cell carcinoma. We studied the prevalence of oral HPV in healthy individuals and its relative risk factors. ...

متن کامل

Survival rate and prognostic factors of esophageal cancer in east Azerbaijan province, North-west of Iran.

BACKGROUND Esophageal cancer in Iran is the sixth most common cancer and is particularly important in east Azerbaijan. The aim of this study was to calculate survival rates and define prognostic factors in esophageal cancer patients. METHODS In this study, all patients with esophageal cancer registered in the Radiation Therapy Center, during March 2006 to March 2011, were analyzed and followe...

متن کامل

Sources of stress for nurses in neonatal intensive care units of East azerbaijan province, iran.

INTRODUCTION Stress is one of the main factors affecting one's efficiency as well as staff health and quality of nursing services. Neonatal intensive care units (NICUs) can be stressful environments for nurses, infants and families as well. Since there is no evidence in this regard in Iran, the present study aimed to determine stress levels related to care delivering in NICU from the viewpoint ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 68  شماره 2

صفحات  125- 129

تاریخ انتشار 2013-11-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023